DSpace Collection: MCB
http://localhost:8080/xmlui/handle/123456789/23
MCB2023-01-01T17:24:09ZPHYSIO-CHEMICAL AND MICROBIAL EVALUATION OF MTU FISH POND WASTE WATER
http://localhost:8080/xmlui/handle/123456789/1119
Title: PHYSIO-CHEMICAL AND MICROBIAL EVALUATION OF MTU FISH POND WASTE WATER
Authors: ACHIGBUE, ANTHONY IFEANYI
Abstract: Pollution from pond farm wastewater entering surrounding drainage systems and water bodies is common in the Nigerian community, and might lead to water borne diseases as well as adverse health related problems. This study was designed to assess the presence of Vibrio spp and Escherichia coli in waste water from three Mountain Top University Fish Ponds waste water samples. Identification and characterization of Vibrio spp and E. coli in the waste water samples collected weekly over a period of one month were carried out using morphological, biochemical and molecular tests. In addition, Physico-chemical analysis of the waste water samples was carried out to determine temperature, pH, salinity, conductivity, dissolved oxygen (DO), biological
oxygen demand (BOD) levels of the water sample. The morphological test shows that vibrio was positive when cultured on TCBS agar and E.coli when cultured on SMAC agar, showing their morphological features, for the biochemical test Vibrio was positive for catalase, oxidase, Starch hydrolysis, and citrate but was negative for Gram staining. At the molecular level, multiplexed PCR showed the presence of Vibrio cholera at 302bp (ompW (Vibrio cholera)-304bp). The physicochemical parameters were within standard permissible limit. These findings suggest that the fish pond effluent contains possible pathogens, therefore it is critical to monitor it quality before disposing it into the ecosystem. The presence of these organisms may be a sign that the
water samples has become contaminated, and when expose to human ecosystem could cause certain water-borne disease2022-09-01T00:00:00ZSTUDY OF MICROBIAL LOADS IN WATER AND AIR SAMPLES FROM OGUN STATE DUMPSITE
http://localhost:8080/xmlui/handle/123456789/1111
Title: STUDY OF MICROBIAL LOADS IN WATER AND AIR SAMPLES FROM OGUN STATE DUMPSITE
Authors: EGBETOKUN, SAMUEL OLUWASEYI
Abstract: In this study, the settled plate method was used to measure the microbial loads in air and water samples taken from the Shagamu dumpsite in Ogun State. The analysis procedure comprises exposing sterile potato dextrose agar (PDA) and nutritional agar (NA) and NA to the air for 0 to 30 minutes. Additionally sampled and transported to the lab for study was water from a nearby river. Colonies that could be seen were counted and noted in water samples and exposed air plates. The isolates underwent morphological and biochemical characterizations. Testing the isolated bacteria for antibiotic sensitivity was also done. The colony count after 30 minutes of plates exposure for nutrient agar (NA) and (PDA) potato dextrose agar ranged from 27 – 400 cfu and 73 – 1400 cfu respectively. The highest counts was observed at 30 minutes of exposure while the lowest was at 0 minutes. The colony count after 48 hours of incubations ranged from 150x103 - 45x105 and 300x103 - 130x105 for the first and second wastewater sampling respectively. Escherichia coli, S. aureus, Micrococcus sp., Pseudomonus spp., and Bacillus spp., were the predominant bacteria. Antibiotics sensitivity testing carried out on the isolated bacteria showed that 27% were resistant to the selected antibiotics diskIn conclusion, our study's observations of the landfill waste disposal and its close vicinity to neighborhoods suggest that there may be a serious environmental health risk that could have a serious impact on people's health and safety. To maintain public safety and to control waste management, the government must enact policies.2022-09-01T00:00:00ZCHARACTERIZATION OF staphylococcus aureus ISOLATED FROM DOOR HANDLES IN THE COLLEGE OF HUMANITIES, MANAGEMENT AND SOCIAL SCIENCES, MOUNTAIN TOP UNIVERSITY
http://localhost:8080/xmlui/handle/123456789/1109
Title: CHARACTERIZATION OF staphylococcus aureus ISOLATED FROM DOOR HANDLES IN THE COLLEGE OF HUMANITIES, MANAGEMENT AND SOCIAL SCIENCES, MOUNTAIN TOP UNIVERSITY
Authors: UMOH, HARVEY PAUL
Abstract: Staphylococcus aureus is a gram-positive organism that causes diseases and infections in humans, in order to ascertain the prevalence of Staphylococcus aureus on door handles and the organism sensitivity to commonly used antibiotics in the College of Humanities Management and Social Sciences, Mountain Top University, A study was conducted. A total of 30 swab samples were collected from door handles, 10 each from restrooms,
office doors, and lecture halls of the College of Humanities Management and Social Sciences at Mountain Top University. Using a combination of morphological characteristics on agar and biochemical tests, the samples were cultivated and S. aureus identified. Staphylococcus aureus was isolated from a total of 16 (53%) of the 30 samples collected. Results from the susceptibility testing showed that Tetracycline was the most effective against the S. aureus isolates (14 isolates were seen to be susceptible). Findings from this study demonstrate that Staphylococcus aureus, which is capable of infecting people, is present on the door handles. Serious health issues may result from these microbes. Therefore, maintaining excellent personal hygiene practices like washing your hands frequently will help to lower the risk of microbial transmission.2022-09-01T00:00:00ZMOLECULAR DETECTION OF NOROVIRUS IN CHILDREN PRESENTING WITH DIARRHEA IN OGUN STATE, NIGERIA
http://localhost:8080/xmlui/handle/123456789/1091
Title: MOLECULAR DETECTION OF NOROVIRUS IN CHILDREN PRESENTING WITH DIARRHEA IN OGUN STATE, NIGERIA
Authors: OLAWALE, EUNICE AYOMIDE
Abstract: Norovirus (NoV) is amongst the eminent etiological agent of viral gastroenteritis outbreaks
worldwide. Apart from rotavirus, NoV plays a rising and crucial role in enteric infection in
children, particularly during the post-rotavirus vaccination period. Among the three human
norovirus strains discovered, GII genogroup is more prevalent in Nigeria and they also occur during the dry season. Children infected with NoV typically exhibit classic clinical signs of acute viral gastroenteritis, such as vomiting and liquid stools, and paediatric patients are more likely to develop dehydration that necessitates hospitalisation. Although most NoVs infection symptoms are self-limiting, recurring infection is not uncommon in children. However, its incidence is rarely reported in developing countries such as Nigeria. The aim of this study was to investigate the prevalence of Norovirus in children below the age of five presenting with diarrhea in Ogun State, Nigeria. With the consent of the caretakers, a questionnaire was provided, and 100 stool samples were obtained from children who had diarrhoea. Viral RNA was isolated from the samples using molecular methods and tested for the presence of Norovirus using RT-Nested PCR. Samples were amplified by PCR using specific primers to make the result more legible. The collected data were statistically analysed using SPSS version 20. Analysis of the samples showed the prevalence of Norovirus to be 6%, of which 4% were male and 2% were female. Subsequent investigation found that children under the age of two were more impacted than children beyond the age of two. This, however, was not statistically relevant. The P-value of > 0.05 is significant. In conclusion, this study established the importance of NoV as a cause of paediatric diarrhoea and identified norovirus as one of the common causative agents of paediatric illness in circulation in Ogun State, Nigeria. Norovirus gastroenteritis is a public health hazard that requires quick action to prevent future spread.2022-09-01T00:00:00Z