DSpace Collection: BCH
http://localhost:8080/xmlui/handle/123456789/9
BCH2023-01-01T17:19:26ZANTI-INFLAMMATORY EFFECT OF ALPHA LIPOIC ACID AND OLIVE OIL ON LETROZOLE-INDUCED POLYCYSTIC OVARIAN SYNDROME IN RAT MODEL: A COMPARATIVE STUDY
http://localhost:8080/xmlui/handle/123456789/1067
Title: ANTI-INFLAMMATORY EFFECT OF ALPHA LIPOIC ACID AND OLIVE OIL ON LETROZOLE-INDUCED POLYCYSTIC OVARIAN SYNDROME IN RAT MODEL: A COMPARATIVE STUDY
Authors: IGENE, PRECIOUS EVY
Abstract: Polycystic ovarian syndrome (PCOS) is a multi-symptom endocrine disorder that results from androgen excess, particularly testosterone, and ovarian dysfunction that disrupts HPO axis function. Women with PCOS possess an altered immune response system which presents a low-grade chronic inflammatory state in them. Serum cytokine levels in the letrozole-induced PCOS rats will be investigated after treatment with Alpha lipoic acid and Olive oil and the results compared with a healthy control group. Thirty-five rats were used for this experiment,
each group consisting of seven rats. Group 1 was the control group, while Groups 2 – 5 were induced with 1ml/1kg of letrozole for 22 days. Drug administration for the rats distributed into five groups are as follows: the non-PCOS control group was administered 0.5ml of saline water, the PCOS control group was administered 0.5ml of distilled water, and three PCOSinduced groups were administered 2mg/kg of clomiphene citrate and 7.14mg/kg of metformin, 1mg/kg of alpha lipoic acid and 4ml/kg of olive oil respectively for 14 days. At
the end of the experimental period, the rats were anaesthetized with diethyl ether and sacrificed by jugular puncture 25 hours after the last treatment. The serum Tumor Necrosis Factor (TNF-𝛼) and Interleukin (IL-6) levels of letrozole-induced polycystic ovarian syndrome rats were assayed for and the results were analyzed. It was reported in this study, that letrozole-induced PCOS Wistar rats possess high serum levels of TNF- 𝛼 and IL-6. Alpha lipoic acid and Olive oil exert anti-inflammatory potentials against IL-6 released into the bloodstream compared favourably with metformin and clomiphene citrate. Olive oil showed a great anti-inflammatory potential against TNF- 𝛼 release into the bloodstream. Therefore, it can be exploited as a management option in the treatment of PCOS.2022-09-01T00:00:00ZEFFECTS OF DIABETES AND Plasmodium berghei INFECTION ON SOME IMMUNE AND BIOCHEMICAL PARAMETERS IN BALB/c MICE
http://localhost:8080/xmlui/handle/123456789/1027
Title: EFFECTS OF DIABETES AND Plasmodium berghei INFECTION ON SOME IMMUNE AND BIOCHEMICAL PARAMETERS IN BALB/c MICE
Authors: ADELEKE, WISDOM ADEOLA
Abstract: Diabetes is a chronic, metabolic disease characterized by elevated levels of blood glucose (or blood sugar), which leads over time to serious damage to the heart, blood vessels, eyes, kidneys, and nerves. Malaria is a life-threatening disease caused by protozoan parasites of the Plasmodium species that are transmitted by infected mosquitoes. Diabetes and Malaria are prevalent diseases in Nigeria. Malaria has been documented to be more common in diabetes, therefore it is essential to investigate the effects of the co-morbid conditions on immune and biochemical parameters.
Fifty- four male BALB/C mice were used in this study. The mice were grouped into eight groups, Group 1 (Normal control), Group 2 (Malaria only), Group 3 (Diabetes only), Group 4 (Diabetes + Malaria), Group 5 (Malaria + AL), Group 6 (Diabetes + MTF), Group 7 (Malaria + Diabetes + MTF) and Group 8 (Malaria + Diabetes + MTF + AL). Diabetes was induced using Streptozotocin (40 mg/kg) for 5 consecutive days while malaria was established by inoculating each mice with 0.2 mL of Plasmodium berghei. 200 mg/kg body weight of Metformin and 1.14/ 6.86 mg/kg body weight of Artemether Lumefantrine were used for treatment of diabetes and malaria respectively. This study showed a significant decrease in creatinine concentration of infected groups with treatment when compared to negative control groups. The results reveal that there was no significance in the Urea Concentration in the Control Groups (Diabetes Only, Malaria Only, Malaria + Diabetes Only) when compared across all other groups. The result of serum levels of TNF –α conducted in experimental mice revealed that there is no significant increase in the median concentration of TNF –α across all the groups when compared. The result of serum levels of interleukin-6 conducted in experimental mice revealed that there was a significant increase in IL-6 Concentration (p=0.046) in group 4 (Malaria + Diabetes Only) when compared with the Normal Control. The findings of this study provides evidence for increased risk for P. berghei infection in Diabetes and also showed that Metformin does not have a prophylactic effect on malaria treatment.2022-09-01T00:00:00ZEVALUATION OF GENOTOXIC AND BIOCHEMICAL EFFECTS OF SOFT DRINK (BIGI COLA AND ORANGE) USING Allium cepa AND Drosophila melanogaster BIOASSAYS
http://localhost:8080/xmlui/handle/123456789/1017
Title: EVALUATION OF GENOTOXIC AND BIOCHEMICAL EFFECTS OF SOFT DRINK (BIGI COLA AND ORANGE) USING Allium cepa AND Drosophila melanogaster BIOASSAYS
Authors: PETRA, VERA ESTHER
Abstract: The genotoxicity of carbonated soft drinks were investigated using the Allium cepa and Drosophila assay, this present practical has been conducted to study the genotoxic effect of three concentrations of Bigi drinks “Cola and Orange” with concentrations (6.25, 12.5 and 25) respectively with time exposure of 72hours and root growth inhibition test is performed on root tips of Allium cepa while pipe borne water served as negative control and was also exposed for 72hours, lead nitrate [PbNO3]2 served as positive control and was exposed for 72hours.
However, the toxic effect of soft drinks on Drosophila melanogaster was conducted with three concentrations of Bigi drinks “Cola and Orange” {100, 50 &25} with time exposure of 10 days in each vials having triplicate of each group and negative control, negative control which is the distilled water that was exposed for 10 days. Unfortunately, promising results were not obtained from the Allium cepa assay.
This conducted research on assessment of the genotoxic effects of soft drinks by bioassay methods will make it possible to extend the understanding of the processes and mechanisms of this toxicity and form more rational concept of consumption.2022-09-01T00:00:00ZMETABOLISM OF AMINO ACIDS AND PROTEINS
http://localhost:8080/xmlui/handle/123456789/957
Title: METABOLISM OF AMINO ACIDS AND PROTEINS
Authors: Ayodele, O. O
Description: METABOLISM OF AMINO ACIDS AND PROTEINS (BCH 311)2021-01-01T00:00:00Z