EVALUATION OF GENOTOXIC AND BIOCHEMICAL EFFECTS OF SOFT DRINK (BIGI COLA AND ORANGE) USING Allium cepa AND Drosophila melanogaster BIOASSAYS

Abstract

The genotoxicity of carbonated soft drinks were investigated using the Allium cepa and Drosophila assay, this present practical has been conducted to study the genotoxic effect of three concentrations of Bigi drinks “Cola and Orange” with concentrations (6.25, 12.5 and 25) respectively with time exposure of 72hours and root growth inhibition test is performed on root tips of Allium cepa while pipe borne water served as negative control and was also exposed for 72hours, lead nitrate [PbNO3]2 served as positive control and was exposed for 72hours. However, the toxic effect of soft drinks on Drosophila melanogaster was conducted with three concentrations of Bigi drinks “Cola and Orange” {100, 50 &25} with time exposure of 10 days in each vials having triplicate of each group and negative control, negative control which is the distilled water that was exposed for 10 days. Unfortunately, promising results were not obtained from the Allium cepa assay. This conducted research on assessment of the genotoxic effects of soft drinks by bioassay methods will make it possible to extend the understanding of the processes and mechanisms of this toxicity and form more rational concept of consumption.