THE ADVENT MOLECULAR TECHNIQUES IN THE IDENTIFICATION OF RHIZOBACTERIA

Abstract

The rhizosphere is the most productive part of the soil in which the biogeochemical cycle of organic matter and mineral nutrients is mainly introduced and affected, as well as a host of landscape and global processes. In view of the role of these bacteria specifically in plant growth in the rhizosphere, it is important to know the exact identity of the (beneficial) rhizobacteria involved before applying for plant growth and development. Conventional method for identification of rhizobacteria have long time been considered as the most accurate until recently that was partially faulted due to advent of molecular techniques. As a consequence of advancement in molecular techniques, numerous questions been answered by the provision of more accurate information about bacteria in their natural habitat, As well as the refining of microbial ecology, this led to the generation of newer questions about the roles and functions of bacteria in the rhizosphere. Going ahead in the fields of microbial taxonomy and systematics, science needs to discuss more thoroughly the problems of clustering, population genomics and the development of innovative approaches to classification and characterization. Therefore, correct identification of rhizobacteria is of fundamental importance to sustainable agricultural microbiology as well as to scientists involved in many other areas of applied research and industry such as pharmaceutical microbiology, microbial biotechnology, food production and climate change. In all, accurate identification requires a sound classification or system of ordering organisms into groups, as well as an unequivocal nomenclature for naming them.