PATHOGENIC E. COLI SEROTYPES ISOLATED FROM STREET VENDED FRESH PRODUCE IN IBAFO, OGUN STATE.

Abstract

Fresh produce, mostly fruits and vegetables, is an important part of the human diet since it contains important vitamins, minerals, and fiber. Increased consumption of fresh food, on the other hand, has contributed to an increase in the frequency of disease outbreaks around the world. Because fresh produce is frequently consumed raw, it is more likely to be contaminated with pathogens such as E. coli 0157:H7, which can cause food poisoning and, in extreme circumstances, renal failure and death, especially in children. Fresh produce is purchased in both closed stores and roadside open kiosks in impoverished nations like Nigeria, and most of the time it is not treated hygienically. The prevalence of Shiga toxin-producing E. coli in various fresh produce (Lettuce, Watermelon, Pineapple, Cucumber, Cabbage, Carrot, and Pawpaw) from various roadside fruit vendors in different locations around the South-west region of Nigeria, specifically Lagos and Ogun state, is reported in this study. Using SMAC and MAC plates, a total of 64 samples were tested for E. coli. STEC virulence genes (stx1, stx2, and eae) were identified using multiplex PCR in selected isolates (n = 21). The presumptive STEC total viable count (TVC) in the samples ranged from 4.5 to 7.8 log10cfu/g. Ibafo lettuce had the greatest total viable count of 7.8 log10cfu/g. The existence of STEC was confirmed by the presence of the stx1 virulence gene detected only in Magboro Watermelon, as determined by Multiplex PCR. Foodborne disorders such as haemorrhagic colitis (HC) or haemolytic uremic syndrome (HUS) are all risks associated with the presence of STEC. The presence of STEC in fresh produce is of public health concerns, Therefore, awareness in terms of hygiene needs to be made for the consumers.