ANTIOXIDANT EFFECT OF Crassocephalum rubens (Jacq.S. Moore) Asteraceae IN ISOPRENALINE INDUCED MYOCARDIAL INFRACTED RATS

Abstract

Myocardial Infarction is a prevalent disorder that leads to the heart failure function, affecting male and female. It is a medical condition that arises when a blood clot completely obstructs the coronary artery leading to death of tissue, which causes a lasting injury to the heart muscle. Crassocephalum rubens has been reported to possess an array of medicinal properties, with the leaves being used to treat indigestion, headaches, bruises, nosebleeds and sleeping sickness. The reported pharmacological activities of C. rubens include: anticoagulant properties that can be used in the treatment of clotting disorders. Other activities are: antidiabetic, anti-inflammatory and antioxidant. The present study evaluated the antioxidant effect of Crassocephalum rubens leaf extracts on myocardial infarction induced by isoprenaline (ISO) in Wistar albino rats with the view to determine its probable use in the management of heart -related diseases. Fresh plant leaves were collected, identified, extracted, fractionated and the methanol extracts were partitioned with ethyl acetate and hexane. Sixty adult rats were divided into (12) groups of 5 rats each. Groups I, II, and III were the control groups. Rats in group IV, V and VI were pretreated with 100, 150, and 200 mg/kg of the crude methanol extract; VII, VIII, IX were pretreated with 100, 150, and 200 mg/kg of hexane fraction; X, XI and XII, were pretreated with 100,150, and 200 mg /kg of Ethyl acetate fraction for 14 days. Myocardial infarction was then induced in all rats (except those in Group I) with a single intraperitoneal injection of ISO (100 mg /kg). Afterwards, the rats were sacrificed. Experiment carried out gave results of a significant increase in lipid peroxidation levels in myocardial –induced rats treated with isoprenaline, there was also an increase in the heart tissue of GSH of myocardial infracted induced rats. The study has shown that C. ruben may be able to reduce the effect of oxidative stress induced myocardial infarction.