ANTI-INFLAMMATORY AND ANTIOXIDANT EFFECTS OF Phyllanthus amarus IN CARRAGEENAN INDUCED INFLAMMATION IN MICE

Abstract

Inflammation is a pathological state that develops from immunological, vascular, and inflammatory cell responses to infection or damage, and is characterized at the tissue level by redness, swelling, heat, discomfort, and loss of tissue function. This can result in DNA damage, tissue death, and interior scarring, all of which have been related to the onset of a variety of disorders. The difference between the formation of reactive oxygen species (ROS) and their elimination by protective systems is known as oxidative stress, and it can lead to chronic inflammation. Many chronic diseases are caused by oxidative stress-induced inflammation. The effects of P. amarus extract on paw oedema, lipid profile, and antioxidant capabilities in carrageenan-induced inflammatory mice were investigated in this work. For seven days, forty-five mice were separated into nine groups and given varied concentrations of P. amarus extract and fractions. Normal saline was administered to the normal control mice, whereas the positive control mice were given the standard medication (Diclofenac). Using Randox kits, the lipid profile and amounts of antioxidant enzymes [catalase, superoxide dismutase (SOD), and reduced glutathione (GSH)] were measured in the mice's serum and liver. P. amarus extract reduced serum and liver total cholesterol, triglycerides, VLDL-C, and LDL-C significantly (p< 0.05), whereas serum HDL-C was significantly raised. SOD, Catalase activity, and Glutathione concentration all increased significantly (p<0.05) in P. amarus.This study showed that P. amarus exerts a hypolipidemic and antioxidant effects which improves the lipid profile of the inflammatory mice.