SCREENING AND PRODUCTION OF LIPASE FROM Streptomyces sp

Abstract

Lipases (EC 3.1.1.3) is a triacylglycerol acylhydrolase that works on carboxylic ester linkages. They belong to the class of serine hydrolases and do not require any cofactor. In this study, bacterial isolates from domestic wastewater contaminated soils were screened on solid agar for lipase production. The best isolate was identified using morphological and biochemical characterizations. Effects of physico-chemical parameters such as carbon and nitrogen sources, temperature, and pH on lipase production were investigated. A total of 15 bacterial species were isolated and screened for lipase production. Isolate PSN07 showed the highest lipase activity and was identified as Streptomyces sp. Carbon sources, nitrogen sources, incubation temperature and initial pH had significant effects (p<0.05) on lipase production. Glucose (65.95a U/mL), yeast extract (88.99a U/mL), 30oC (69.41a U/mL) and pH 6.0 (80.64a U/mL) supported the optimum lipase production by the selected isolate. Streptomyces sp. PSN07 isolated from domestic wastewater contaminated soil was a good lipase producer which can be developed for the industrial production of biotechnologically important products such as food, beverages, leather, textiles, detergents, and soaps.