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Title: | Bio-Kinetics of Simultaneous Nitrification and Aerobic Denitrification (SNaD) by a CyanideDegrading Bacterium Under Cyanide-Laden Conditions |
Authors: | Mpongwana, Ncumisa Ntwampe, Seteno Karabo Obed Omodanisi, Elizabeth I Chidi, Boredi Silas Razanamahandry, Lovasoa C Dlangamandla, Cynthia Mukandi, Melody Ruvimbo |
Keywords: | aerobic denitrification; bio-kinetics; free cyanide; nitrate-nitrogen; nitrification; nitrite-nitrogen |
Issue Date: | 14-Jul-2020 |
Publisher: | Journal of Applied Sciences |
Citation: | Mpongwana, N.; Ntwampe, S.K.O.; Omodanisi, E.I.; Chidi, B.S.; Razanamahandry, L.C.; Dlangamandla, C.; Mukandi, M.R. Bio-Kinetics of Simultaneous Nitrification and Aerobic Denitrification (SNaD) by a Cyanide- Degrading Bacterium Under Cyanide-Laden Conditions. Appl. Sci. 2020, 10, 4823. https://doi.org/10.3390/app10144823 |
Series/Report no.: | 10; |
Abstract: | A microorganism isolated and identified as Acinetobacter courvalinii was found to be able to perform sequential free cyanide (CN−) degradation, simultaneous nitrification and aerobic denitrification (SNaD); this ability was associated with the multiphase growth profile of the microorganism when provided with multiple nitrogenous sources. The effect of CN− on SNaD including enzyme expression, activity and protein functionality of Acinetobacter courvalinii was investigated. It was found that CN− concentration of 1.9 to 5.8 mg CN−/L did not affect the growth of Acinetobacter courvalinii. Furthermore, the degradation rates of CN− and ammonium-nitrogen (NH4-N) were found to be 2.2 mg CN−/L/h and 0.40 mg NH4 -N/L/h, respectively. Moreover, five models’ (Monod, Moser, Generic Rate law, Haldane, and Andrews) ability to predict SNaD under CN− conditions, indicated that, only the Rate law, Haldane and Andrew’s models, were suited to predict both SNaD and CN− degradation. The effect of CN− on NH4-N, nitrate-nitrogen (NO3 −) and nitrite-nitrogen (NO2 −) oxidizing enzymes indicated that the CN− did not affect the expression and activity of ammonia monooxygenase (AMO); albeit, reduced the expression and activity of nitrate reductase (NaR) and nitrite reductase (NiR). Nevertheless, a slow decrease in NO2 − was observed after the supplementation of CN− to the cultures, thus confirming the activity of NaR and the activation of the denitrification pathway by the CN−. These special characteristics of the Acinetobacter courvalinii isolate, suggests its suitability for the treatment of wastewater containing multiple nitrogenous compounds in which CN− is present |
URI: | http://localhost:8080/xmlui/handle/123456789/446 |
Appears in Collections: | Microbiology |
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applsci-10-04823pdf.pdf | 1.75 MB | Adobe PDF | View/Open |
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