EVALUATION OF THE EFFECTS OF HYDROMETHANOL EXTRACT AND FRACTIONS OF Crassocephalum rubens ON BLOOD COAGULATION AND CARDIAC PARAMETERS IN ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTED RATS

Abstract

Myocardial infarction is a serious cardiac condition that is considered a significant reason for death and morbidity across the world. Atherosclerosis, or the emergence of blood clots in the coronary arteries over time, is the reason. Blood coagulation is a fast and effective process that results in clot formation that needs control. The studies on the effects of hydromethanol extract and fractions Crassocephalum rubens (C. rubens) against blood coagulation profile and concentration of cardiac markers induced by drugs and therapeutic agents remain insufficient, especially its effects on Isoproterenol (ISO.) Special emphasis was given to the impact of C. rubens in blood coagulation cascade and cardiac enzymes concentration where sixty adult male albino rats were used in this study. The animals were divided into twelve equal groups at random. Group I served as control and received 1ml phosphate buffer saline. Group II received ISO and aspirin orally, Group III received ISO without pretreatment, followed by intraperitoneal injection of ISO (100 mg/kg), to induce myocardial infarction. Group IV-XII received C. rubens extracts and fractions at different doses -100mg/kg, 150mg/kg and 200mg/kg. Plant administration was done by oral gavage daily for 14 days, followed by MI induction on day 15. The result of showed significant increase in the blood coagulation profile (clotting and prothrombin time) and elevation of cardiac markers (alkaline phosphatase and total protein) in the plasma and decrease in the bleeding time, activated partial thromboplastin time and Lactate dehydrogenase concentration when compared to the normal control group, with the exception of certain concentrations of the plant extract which indicates that C.rubens has procoagulant activity.