dc.contributor.author |
Mpongwana, Ncumisa |
|
dc.contributor.author |
Ntwampe, Seteno Karabo Obed |
|
dc.contributor.author |
Omodanisi, Elizabeth I |
|
dc.contributor.author |
Chidi, Boredi Silas |
|
dc.contributor.author |
Razanamahandry, Lovasoa C |
|
dc.contributor.author |
Dlangamandla, Cynthia |
|
dc.contributor.author |
Mukandi, Melody Ruvimbo |
|
dc.date.accessioned |
2022-07-04T10:34:00Z |
|
dc.date.available |
2022-07-04T10:34:00Z |
|
dc.date.issued |
2020-07-14 |
|
dc.identifier.citation |
Mpongwana, N.; Ntwampe, S.K.O.; Omodanisi, E.I.; Chidi, B.S.; Razanamahandry, L.C.; Dlangamandla, C.; Mukandi, M.R. Bio-Kinetics of Simultaneous Nitrification and Aerobic Denitrification (SNaD) by a Cyanide- Degrading Bacterium Under Cyanide-Laden Conditions. Appl. Sci. 2020, 10, 4823. https://doi.org/10.3390/app10144823 |
en_US |
dc.identifier.uri |
http://localhost:8080/xmlui/handle/123456789/446 |
|
dc.description.abstract |
A microorganism isolated and identified as Acinetobacter courvalinii was found to be able
to perform sequential free cyanide (CN−) degradation, simultaneous nitrification and aerobic
denitrification (SNaD); this ability was associated with the multiphase growth profile of the
microorganism when provided with multiple nitrogenous sources. The effect of CN− on SNaD
including enzyme expression, activity and protein functionality of Acinetobacter courvalinii was
investigated. It was found that CN− concentration of 1.9 to 5.8 mg CN−/L did not affect the growth
of Acinetobacter courvalinii. Furthermore, the degradation rates of CN− and ammonium-nitrogen
(NH4-N) were found to be 2.2 mg CN−/L/h and 0.40 mg NH4
-N/L/h, respectively. Moreover, five
models’ (Monod, Moser, Generic Rate law, Haldane, and Andrews) ability to predict SNaD under
CN− conditions, indicated that, only the Rate law, Haldane and Andrew’s models, were suited to
predict both SNaD and CN− degradation. The effect of CN− on NH4-N, nitrate-nitrogen (NO3
−)
and nitrite-nitrogen (NO2
−) oxidizing enzymes indicated that the CN− did not affect the expression
and activity of ammonia monooxygenase (AMO); albeit, reduced the expression and activity of
nitrate reductase (NaR) and nitrite reductase (NiR). Nevertheless, a slow decrease in NO2
− was
observed after the supplementation of CN− to the cultures, thus confirming the activity of NaR
and the activation of the denitrification pathway by the CN−. These special characteristics of the
Acinetobacter courvalinii isolate, suggests its suitability for the treatment of wastewater containing
multiple nitrogenous compounds in which CN− is present |
en_US |
dc.description.sponsorship |
Ncumisa Mpongwana, Seteno Karabo Obed Ntwampe, Elizabeth Ife Omodanisi , Boredi Silas Chidi , Lovasoa Christine Razanamahandry, Cynthia Dlangamandla and Melody Ruvimbo Mukandi |
en_US |
dc.language.iso |
en_US |
en_US |
dc.publisher |
Journal of Applied Sciences |
en_US |
dc.relation.ispartofseries |
10; |
|
dc.subject |
aerobic denitrification; bio-kinetics; free cyanide; nitrate-nitrogen; nitrification; nitrite-nitrogen |
en_US |
dc.title |
Bio-Kinetics of Simultaneous Nitrification and Aerobic Denitrification (SNaD) by a CyanideDegrading Bacterium Under Cyanide-Laden Conditions |
en_US |
dc.type |
Article |
en_US |