Interaction of titanium dioxide and zinc oxide nanoparticles induced cytogenotoxicity in Allium cepa

Abstract

The extensive production and utilisation of titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles (NPs) in consumable items may enhance signifcant increase in fauna and fora exposure. Studies showing the interactive efect of NPs in biological systems are limited. Herein, we showed the cytogenotoxic efects of TiO2 and ZnO NPs, and their mixture (1:1) using the Allium cepa assay. Mitotic index (MI) and chromosomal aberrations (CAs) were assessed in A. cepa L. bulbs exposed to each NP and their mixture at concentrations of 5, 10, 20, 40 and 80 mg L−1, respectively. The recovery efect of the root tip cells from the cytogenotoxic efects of the nanoparticles was also investigated. TiO2, ZnO NPs and their mixture significantly (p<0.05) induced increase in CA and reduction in MI in A. cepa root cells, but the mixture induced the highest frequency of CA and reduction in MI. When the treated meristematic cells were placed in water for recovery, there were reduction in the number of aberrant cells in A. cepa exposed to TiO2 and the mixture. Interactive factor analysis of the efects of the mixture showed antagonism. The aberrations induced by TiO2 NPs appeared to be transient while those induced by ZnO NPs may be transmissible due to the increase in frequency of aberrations in the recovery test. This fnding sho